🧬 Enzyme Kinetics

Enzyme

[S] Substrate: 10.0 mM

[E] Enzyme: 1.0x

Temp: 37°C

pH: 7.0

Inhibitor

Lineweaver-Burk Plot

Kinetics

V = 28.571 mol/s

Vmax = 100.00

Km = 25.00 mM

V = Vmax·[S] / (Km + [S])

Michaelis-Menten

V = Vmax x [S] / (Km + [S]). Vmax is the maximum rate at saturating substrate. Km is the substrate concentration at half Vmax - lower Km = higher affinity. Competitive inhibitors raise Km, non-competitive lower Vmax.